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| 猪熊, 壽; 永田, 美和; 細井, 栄嗣; 板本, 和仁; 奥田, 優; INOKUMA, Hisashi. |
| 全国8地域のニホンジカに寄生するタイレリアの18S rRNAおよびp33/34遺伝子を解析した. 18S rRNA遺伝子の相同性は99.7-100%であったが,山口県産ニホンジカのタイレリアp33/34遺伝子の相同性は,他地域のものに比べて塩基配列で68.7〜70.1%,アミノ酸配列で64,1〜70.0%と低く,ニホンジカのタイレリアには遺伝的に異なる株が存在することが示唆された. The 18S rRNA gene and the piroplasm major immunodominant protein gene (p33/34) of Theileria from various subspecies of sika deer in 8 different locations of Japan were analyzed. The similarity between 633 bp partial sequences of the 18S rRNA gene among various subspecies of sika deer was found to be between 99.7% and 100%. While the percent identities of the 412 bp partial p33/34 gene sequence and deduced amino acid sequences between Theileria of sika deer from Yamaguchi Prefecture and those found in deer from other Prefectures, were comparatively low, 68.7% to... |
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Palavras-chave: Cervue nipon; Divergence; Theileria sp. |
| Ano: 2008 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4004 |
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| 久保田, 直樹; 坂田, 義美; 宮崎, 直美; 板本, 和仁; 坂内, 天; 西川, 義文; 玄, 学南; 猪熊, 壽; NISHIKAWA, Yoshifumi; XUAN, Xuenan; INOKUMA, Hisashi. |
| 組換抗原を用いた種特異的ELISAにより,犬のNeospora caninum感染について全国的血清疫学調査を実施した.1,206頭中全国30県から126頭(10.4%)が陽性を示した.陽性率は,雄よりも雌で有意に高く,また他品種と比較して有意に高い陽性率を示す品種がみられた.子宮蓄膿症および糖尿病の病歴を有する犬の陽性率は,他の疾病を有する犬または疾病のない犬の陽性率に比べて高値であった. A seroepidemiological survey of Neospora caninum infection among dogs in Japan was conducted using species-specific enzyme-linked immunosorbent assay with recombinant surface antigen (Nc-SAG1t). Among 1,206 dogs examined, 126 dogs (10.4%) from 30 prefectures from Hokkaido to Okinawa were positive to N. caninum infections, which were more frequently detected in females than males. Siberian Huskies showed the highest positive rate compared with the other breeds. Dogs with pyometra and diabetes mellitus showed the higher positive rates than dogs with other... |
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Palavras-chave: Canine; Nbospora caninum; Species-specific ELISA. |
| Ano: 2008 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4003 |
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